Our new Clear+ tissue clearing method is the only process that delipidates samples without adjust in morphology and with minimum impact on structural integrity.
Megatome is often a vibrating microtome intended to segment a wide choice of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With large blade vibrating frequency and minimized blade deflection, Megatome permits substantial-throughput tissue sectioning with uniform floor profile, along with minimal tissue problems and data loss.
Antibodies may possibly consider months to diffuse by means of only some millimeters of tissue, by using a steep labeling gradient from surface to Main.
Megatome is designed for accuracy: the blade vibrates at the next frequency and larger amplitude array than other microtomes, and includes a exceptional deflection Manage system.
SE utilizes a rotational electrical subject to disperse extremely electromobile molecules (for example antibodies or surfactant micelles) throughout a porous sample with no harming electrically charged structures inside the tissue. This permits two-four working day clearing of intact organs,
Our preformulated EasyIndex Alternative raises and homogenize the refractive index of delipidated tissue samples, rendering them fully transparent. This enables mild penetration to the sample and assures the acquisition of substantial-resolution, in-concentration graphic details.
You must only ought to set the javadocTask home when the task you use to create Javadoc is non-common. Such as, there might be a process referred to as allJavadoc within a multi-module
eFLASH is a swift tissue labeling approach that permits for uniform whole-organ staining in twenty rounds of labeling.
The SmartSPIM light-weight sheet microscope supplies unparalleled resolution and acquisition speed all over intact tissue volumes, even though its modular style means that you can up grade and personalize individual parts.
Docset generation calls for at minimum two selections: the name with the docset and the location from the Javadoc information to incorporate inside the docset.
This will likely crank out a feed Listing during the javadoc2dash.outputLocation directory. This Listing will comprise an XML file describing the feed
SHIELD avoids the variability of hydrogel embedding and megatomi.com the knowledge decline from PFA preservation, protecting specimens for several rounds of processing.
--displayName: Will established the identify as shown in Sprint. This can be handy if you develop a docset with identify SampleProject but Display screen name Sample Venture as an alternative. This placing will default to the worth of --identify if omitted.
Our novel SHIELD tissue preservation procedure kinds intramolecular bonds utilizing polyfunctional, versatile epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.